Increased leukocyte infiltration in ischemic muscles of VASP^−/− mice. Hematoxylin and eosin–stained sections of ischemic thigh (A) and calf (F) muscles from WT (+/+) and VASP^−/− (−/−) mice, 7 d after induction of ischemia. Bars, 50 µm. Bar graphs show the quantification of leukocyte numbers isolated from thigh (B–E) and calf (G–J) muscles per gram of tissue before ischemia induction (0) and 3 or 7 d after ischemia induction: neutrophils (B and G), monocytes (C and H), M1-related macrophages (D and I), and M2-related macrophages (E and J). n = 6 animals per group; error bars, SEM; *, P < 0.05; **, P < 0.01; ***, P < 0.001 (two-way ANOVA/Bonferroni).