Wee1 inhibition rescues MyTH4-induced anaphase delay and reduces spindle oscillations. (A) Manually measured orientation of the mitotic spindle versus spindle axis at anaphase onset in cells expressing eGFP-tubulin and mTagBFP-CAAX in the presence of Myo10 morpholino (red and blue traces) or control (gray). Image shows time series of a Myo10-depleted cell expressing BFP-CAAX (blue) and eGFP-α-tubulin (green) and corresponds to the red trace. Time stamps indicate seconds before anaphase (anaphase = 0 s). Bar, 10 µm. (B) Same as A, except cells were treated with PD166285 (red and blue traces) or control (gray). Image shows time series of a PD166285-treated cell expressing mCherry–histone H2B (red) and eGFP–α-tubulin (green) and corresponds to the red trace. (C) Spindle orientation, as a function of spindle orientation at anaphase onset, as determined by the Spindlometer in control (two blue traces) and dynamitin2-CAAX-expressing (six yellow-red traces) embryos. Image shows time series of cells expressing eGFP-tubulin (green), mChe–histone H2B (red), mTagBFP-CAAX (blue), and dynamitin2-CAAX (unlabeled). Bar, 10 μm. (D) Manually measured metaphase duration in cells prepared as in D (control n = 6, Dctn2 n = 13, P = 0.0014).