Cell polarity occurs in the absence of the nucleus. (A) Images and plots showing localization of centrosomes and nuclei from REF52 intact cells and cytoplasts plated on crossbow (intact cells, n = 39; cytoplasts, n = 35) and circle (intact cells, n = 34; cytoplasts, n = 31) patterns. (B) Mean y-coordinate distance ± 95% CI of centrosomes for REF52 intact cells and cytoplasts on crossbow and circle patterns. (C) Images and plots showing localization of Golgi and nuclei from REF52 cells plated on crossbow (intact cells, n = 100; cytoplasts, n = 86) and circle (intact cells, n = 78; cytoplasts, n = 57) patterns. (D) Mean y-coordinate distance ± 95% CI of Golgi for REF52 cells on crossbow and circle patterns. (E) Images and plots showing localization of centrosomes and nuclei from HUVEC intact cells and cytoplasts plated on crossbow (intact cells, n = 52; cytoplasts, n = 57) and triangle (intact cells, n = 50; cytoplasts, n = 49) patterns. (F) Mean y-coordinate distance ± 95% CI of centrosomes for HUVECs on crossbow and triangle patterns. All data are from at least three independent experiments. White dashed line circles in A, C, and E show a 30-µm-diameter region of interest. Bars, 25 µm.