Figure 5.
Figure 5. Mammalian septins recognize membrane curvature. (A) Phalloidin (magenta) and SEPT7 (green) localization, visualized by α-SEPT7 immunofluorescence, in NIH 3T3 fibroblasts. (B) SEPT7 localization (green) in NIH 3T3 fibroblasts treated with 1 µM latrunculin A for 15 min to disrupt actin-dependent septin localization. The cell outline (magenta), was produced by imaging Alexa Fluor 647–conjugated wheat germ agglutinin. (C) 50 nM human septin complexes (SEPT2–SEPT6–SEPT7) labeled with NHS-Alexa Fluor 488 on supported lipid bilayer–coated silica beads. (D) Adsorption of 50 nM human septin complex on bilayer-coated beads (25% PC, 75% PI, and trace RhPE). n ≥ 67 for each size. Dunn test results: ***, P < 0.005; n.s., >0.05. (E) Model displaying the scale of septin curvature sensing compared with other established proteins that interact with curved membranes.

Mammalian septins recognize membrane curvature. (A) Phalloidin (magenta) and SEPT7 (green) localization, visualized by α-SEPT7 immunofluorescence, in NIH 3T3 fibroblasts. (B) SEPT7 localization (green) in NIH 3T3 fibroblasts treated with 1 µM latrunculin A for 15 min to disrupt actin-dependent septin localization. The cell outline (magenta), was produced by imaging Alexa Fluor 647–conjugated wheat germ agglutinin. (C) 50 nM human septin complexes (SEPT2–SEPT6–SEPT7) labeled with NHS-Alexa Fluor 488 on supported lipid bilayer–coated silica beads. (D) Adsorption of 50 nM human septin complex on bilayer-coated beads (25% PC, 75% PI, and trace RhPE). n ≥ 67 for each size. Dunn test results: ***, P < 0.005; n.s., >0.05. (E) Model displaying the scale of septin curvature sensing compared with other established proteins that interact with curved membranes.

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