Curvature preference remains intact in single complexes, but filament formation is required for stable membrane association. (A) Adsorption of 25 nM 6xHIS WT Cdc11–SNAP488 complexes to 1-µm (κ = 2 µm^–1) and 3-µm (κ = 0.67 µm^–1) beads coated in 2% DGS-Ni²⁺ NTA lipids in 100 mM KCl. For visualization of fold enrichment, data were normalized to 3-µm beads. (B) WT Cdc11–SNAP complexes and Cdc11-α6–SNAP complexes, containing point mutations in the polymerization interface of Cdc11, were diluted to 250 nM in 50 mM KCl to promote filament formation and visualized on a polyethylene glycol–coated coverslip. (C) Adsorption of 100 nM 6xHIS Cdc11-α6–SNAP488 complexes to 1-µm (κ = 2 µm^–1) and 3-µm (κ = 0.67 µm^–1) beads coated in 2% DGS-Ni²⁺ NTA lipids in 100 mM KCl. (D) Adsorption of 25 nM 6xHIS WT Cdc11–SNAP488 complexes to 1-µm (κ = 2 µm^–1) and 3-µm (κ = 0.67 µm^–1) beads coated in 2% DGS-Ni²⁺ NTA lipids in 300 mM KCl. (E) WT and α6 mutant Cdc11-GFP expressed and imaged in live A. gossypii. (F) Membrane adsorption of 100 nM WT and Cdc11-α6–SNAP488 complexes on anionic-supported lipid bilayer (25% PC, 75% PI, and trace RhPE)–coated 1-µm beads. In A, C, D, and F, n ≥ 43 for each size. Dunn test results: ***, P < 0.005.