Septin affinity for membranes varies depending on curvature. (A) Adsorption of septins to silica beads over time on 1 µm (κ = 2 µm^–1) and 5 µm (κ = 0.4 µm^–1) at 250-nM septin complex concentration. Solid lines represent means, and shaded areas represent SD (average n/time point: 2 µm^–1 = 158 beads and 0.4 µm^–1 = 18 beads). (B) Adsorption of 500-nM septin complexes on 1-µm (κ = 2 µm^–1) and 5-µm (κ = 0.4 µm^–1) beads in 100 mM KCl. (C) Adsorption of 500-nM septin complexes on 1-µm (κ = 2 µm^–1) and 5-µm (κ = 0.4 µm^–1) bilayer-coated beads in 50 mM KCl. In B and C, n ≥ 50 for each size; black bars represent medians.