Figure 4. GFP::Spn-E dynamics in nuage... | Rockefeller University Press

Figure 4.

$Figure 4. GFP::Spn-E dynamics in nuage is controlled by Qin. (A) A schematic of the FRAP experiment. After prebleach measurement of GFP::Spn-E fluorescence in nuage, one granule was bleached for 62.6 s; thereafter, fluorescence in both reference and bleached nuage was followed over time. Below are representative confocal images in nuage before and after bleaching. Bar, 0.25 µm. (B) Kinetics of GFP::Spn-E fluorescence recovery expressed as a normalized fraction of the prebleach level. Values measured from all replicates are plotted. The fitted curves are single-phase exponential functions. (C) Exponential model parameters estimated from B. (Left) The percentage of prebleached GFP::Spn-E that is mobile within the nuage. Shown are point estimates (horizontal lines) and 99% confidence intervals (shaded boxes) for wild-type and qin mutant mobile fractions. (Right) The recovery rate constants of GFP::Spn-E within the nuage. Point estimates (horizontal lines) and 95% confidence intervals (shaded boxes) are shown. (D) Mean recovery half-time for the mobile fraction of GFP::Spn-E in wild-type and qin mutant nuage. (E) Mean mobile fraction of GFP::Spn-E in bleached nuage of wild-type and qin mutant germline. n = 5 replicates. Error bars are SEM, and p-values are the results of two-tailed t tests.$

GFP::Spn-E dynamics in nuage is controlled by Qin. (A) A schematic of the FRAP experiment. After prebleach measurement of GFP::Spn-E fluorescence in nuage, one granule was bleached for 62.6 s; thereafter, fluorescence in both reference and bleached nuage was followed over time. Below are representative confocal images in nuage before and after bleaching. Bar, 0.25 µm. (B) Kinetics of GFP::Spn-E fluorescence recovery expressed as a normalized fraction of the prebleach level. Values measured from all replicates are plotted. The fitted curves are single-phase exponential functions. (C) Exponential model parameters estimated from B. (Left) The percentage of prebleached GFP::Spn-E that is mobile within the nuage. Shown are point estimates (horizontal lines) and 99% confidence intervals (shaded boxes) for wild-type and qin mutant mobile fractions. (Right) The recovery rate constants of GFP::Spn-E within the nuage. Point estimates (horizontal lines) and 95% confidence intervals (shaded boxes) are shown. (D) Mean recovery half-time for the mobile fraction of GFP::Spn-E in wild-type and qin mutant nuage. (E) Mean mobile fraction of GFP::Spn-E in bleached nuage of wild-type and qin mutant germline. n = 5 replicates. Error bars are SEM, and p-values are the results of two-tailed t tests.