Both For1F-GFP and Sec6-mRuby localize to the developing cell plate during cell division. (A) Images are maximum projections of z-stacks acquired with a laser scanning confocal of a late phragmoplast in a branching cell expressing For1F-GFP and Sec6-mRuby. Sec6-mRuby localizes to prominent intracellular structures, which is particularly evident in branching cells. (B) Images are maximum projections of z-stacks acquired with a laser scanning confocal microscope from a time-lapse image series of a dividing cell. Sec6-mRuby localizes to the phragmoplast throughout cell division, whereas For1F-GFP localizes when the phragmoplast reaches the mother cell wall. Large globular structures in the green channel in A and B are chloroplasts, which autofluoresce under these imaging conditions. Bars, 5 µm. Also see Video 3.