For1F encodes for a protein comprised of an N-terminal Sec10 domain and a C-terminal formin domain. (A) For1F gene model and protein schematic. Top: In the gene model, boxes indicate exons and lines are introns. For both the gene model and the protein prediction, green represents sequences with similarity to Sec10; purple, FH1; blue, FH2; and gray boxes, no known sequence similarity. Numbers indicate amino acid positions. Arrows labeled a and b under the gene model are primers used to perform RT-PCR. (B) RT-PCR of the For1F transcript amplified with primers a and b. Numbers indicate size in kb. (C) Immunoblots performed with an antibody to GFP of cell extracts from a line with For2A (Vidali et al., 2009b) or For1F tagged endogenously with sequences encoding for three tandem GFP molecules. Numbers to the left indicate size in kilodaltons.