Smash is expressed in ectodermal epithelia and muscles. (A–E) WT embryos of the indicated stages were stained with anti–Smash N-term and imaged by confocal microscopy. Left and right images in each row were taken from the same embryo. Images in the left column are superficial optical sections, and images in the right column are cross sections at a deeper focus level. pc, pole cells; ne, neuroectoderm; tr, tracheae; fg, foregut; as, amnioserosa; mt, Malpighian tubule; ep, epidermis; db, denticle belt; sg, salivary gland; vm, visceral musculature; hg, hind gut; ps, posterior spiracle; sm, somatic musculature; pm, pharynx musculature; mtj, myotendinous junction. Bar, 100 µm. Anterior is to the left; dorsal is up.