Flower is localized on intracellular vesicles but not on CGs or on endosomal compartments. (A) SIM images of anti-Flower antibody staining (green) on stimulated WT CTLs transfected with different endosome markers (red, mCherry), CTLs with endogenously labeled CGs from SybKI mice (SybKI CTLs), and WT CTLs costained with anti-GzmB antibody. Bars, 5 µm. (B) Manders’ overlap coefficient of anti-Flower to rab5-, rab7-, rab11-, or syb2-positive compartments. Data are given as mean ± SEM.