Figure 8.
Figure 8. IMM division before OMM division during mitochondrial division. (A) U2OS cells transfected with mito-R-GECO (mitochondrial matrix calcium, red) and GFP-Tom20 (OMM, green) were imaged live by Airyscan microscopy at 9-s intervals after 4 µM ionomycin treatment. Representative time-lapse montage of a mitochondrial division event shown here. Bar, 2 µm. (B) Zoom of the division site (boxed in A). The mito-R-GECO levels have been enhanced to detect the existence of any thin matrix tether. Line scans indicate that the OMM tether persists in the absence of a matrix tether. Bar, 500 nm. (C) Quantification of time between apparent matrix separation and apparent OMM separation in mitochondrial division events after CGP37157 stimulation (example in Fig. S4 E). 29 division events from 23 cells. Mean time, 32.3 ± 29.1 s (SD). Each point represents one division event.

IMM division before OMM division during mitochondrial division. (A) U2OS cells transfected with mito-R-GECO (mitochondrial matrix calcium, red) and GFP-Tom20 (OMM, green) were imaged live by Airyscan microscopy at 9-s intervals after 4 µM ionomycin treatment. Representative time-lapse montage of a mitochondrial division event shown here. Bar, 2 µm. (B) Zoom of the division site (boxed in A). The mito-R-GECO levels have been enhanced to detect the existence of any thin matrix tether. Line scans indicate that the OMM tether persists in the absence of a matrix tether. Bar, 500 nm. (C) Quantification of time between apparent matrix separation and apparent OMM separation in mitochondrial division events after CGP37157 stimulation (example in Fig. S4 E). 29 division events from 23 cells. Mean time, 32.3 ± 29.1 s (SD). Each point represents one division event.

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