Figure 4.
Figure 4. FRAP analysis of GFP-NMIIB in COS-7 cells in the presence or absence of NMIIA. (A–C) Examples of cells expressing GFP-NMIIB alone (A) or GFP-NMIIB together with mCherry-NMIIA (B and C). (Top) Prebleach image of the cell showing GFP-NMIIB (cyan) and mCherry-NMIIA (red). (Bottom) Time frames of the boxed region (15 × 15 µm) before or at indicated times after photobleaching of a ventral stress fiber (A and B) or a transverse arc (C). Bleached regions are marked by a blue box. Bars, 20 µm. (D) FRAP curves corresponding to regions shown in A–C. (E) Halftimes of GFP-NMIIB recovery for indicated conditions. Top and bottom of a box indicate 75th and 25th quartiles, respectively; whiskers encompass all data, the dot is the mean, and and the middle line is the median. Asterisks indicate statistical significance (**, P < 0.01, Dunn’s multiple comparisons test). (F) Individual FRAP data points.

FRAP analysis of GFP-NMIIB in COS-7 cells in the presence or absence of NMIIA. (A–C) Examples of cells expressing GFP-NMIIB alone (A) or GFP-NMIIB together with mCherry-NMIIA (B and C). (Top) Prebleach image of the cell showing GFP-NMIIB (cyan) and mCherry-NMIIA (red). (Bottom) Time frames of the boxed region (15 × 15 µm) before or at indicated times after photobleaching of a ventral stress fiber (A and B) or a transverse arc (C). Bleached regions are marked by a blue box. Bars, 20 µm. (D) FRAP curves corresponding to regions shown in A–C. (E) Halftimes of GFP-NMIIB recovery for indicated conditions. Top and bottom of a box indicate 75th and 25th quartiles, respectively; whiskers encompass all data, the dot is the mean, and and the middle line is the median. Asterisks indicate statistical significance (**, P < 0.01, Dunn’s multiple comparisons test). (F) Individual FRAP data points.

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