PYD aggregates lead to delayed pyroptosis. (A) Live imaging of 3-dpf larvae transiently expressing HSE:PYD_A-mKate2 or HSE:CARD_A-mKate2 with GFP at 17 hphs. Expression of either domain leads to the formation of filamentous aggregates of varying lengths in muscle cells. Time-lapse imaging of asc morpholino-injected Tg(asc:asc-EGFP) larvae transiently expressing the asc morpholino-resistant HSE:PYD*_A–mKate2 (B) or HSE:CARD_A–mKate2 (C) with GFP. If endogenous ASC is absent, PYD*_A aggregates cause cell death 2 h after the aggregates first form, whereas CARD_A aggregates do not, even at 10 h after their assembly. Full time-lapses are included in Video 8. Bars, 40 µm.