Expression of ASC or NLR induces speck formation. Time-lapse imaging of trunk from 3-dpf Tg(asc:asc-gfp) larva transiently expressing HSE:nlr-mKate2 at 7 hphs. (A) Inset shows enlarged view of NLR-mkate2–expressing keratinocytes after speck formation. Full time-lapse is included in Video 2. (B) Time-lapse imaging of 3-dpf Tg(HSE:asc-mKate2) embryos after heat shock. Shown are time points corresponding to 5 hphs (top) and 17 hphs (bottom). (C) Quantification of speck numbers over the entire larvae in five samples imaged overnight after heat shock using three-dimensional image analysis software. (D) Time-lapse of 3 dpf Tg(HSE:asc-mKate2) larvae 3 hphs showing recruitment of ASC-mKate2 to a single speck per cell (demarcated by dashed red line at the time point before speck formation). Both Tg(HSE:asc-mKate2) larvae time-lapses are included in Video 3. (E) Live imaging of Tg(asc:asc-gfp) transiently expressing HSE:asc-mKate2 at 13 hphs. White arrowheads show specks assembled in muscles. (F) Time-lapse imaging of Tg(asc:asc-gfp) larva transiently expressing HSE:asc-mKate2. Individual keratinocytes are demarcated with a dashed white line at the time point before the formation of ASC–mKate2 and ASC–GFP double-positive specks (white arrowheads). Time-lapse was started 3.5 hphs. Full time-lapse is included in Video 3. Bars: (full larvae) 300 µm; (all others) 40 µm.