Endogenous ASC visualized in vivo in the Tg(asc:asc-EGFP) line. Live imaging of Tg(asc:asc-EGFP) for 1-dpf (A), 2-dpf (B), and 5-dpf (C) larvae, with magnification of skin (B′), intestine (C′), and head (C″) optical sections. Live imaging of head, muscle, and caudal hematopoietic tissue (CHT) of 3-dpf Tg(asc:asc-EGFP, spi1b:GAL4,UAS:TagRFP) larvae (D). Bars: (full larvae) 300 µm; (all others) 30 µm.