Tethering KSHV to chromosomes is required for their synthesis. (A) LANA1 and its derivatives are depicted with LANA1/dHBD in which the histone binding domain of LANA1 (HBD; aa 5–23) is deleted or replaced with the AT-hooks of EBNA1. (B) MiniKLacO signals in SLK cells expressing LANA1/AT-hook in two independent clones were followed without selection for 50 generations by FISH. MiniKLacO was lost from SLK/LANA1/AT-hook cells at a rate of 4.6% per generation.