Synthesis and unequal partitioning of KLacO and miniKLacO. (A) The sum of intensities of miniKLacO signals in 24 cells followed for 60 h was determined with CAPS and scaled to 24 h for their progression from G1 through G2, with intensities at the beginning of G1 set to 1 and shown to double during S phase. The temporal scaling necessitated signals being binned; the SE for these bins is shaded in blue. (B) Shown are analyses of 11 cells with the distributions of miniKLacO signals and the sum of their intensities (a.u.) measured by CAPS in G1 through a complete cell cycle to their daughter cells in G1, illustrating their unequal partitioning (daughter cell B was arbitrarily assigned the higher-intensity signals). (C) SLK/wild-type LANA1 cells harboring miniKLacO and expressing the LacI-tdTomato fusion were transduced to express GFP-fused α-tubulin; 12 h after release from a double aphidicolin block, the signals of miniKLacO had different intensities and were distributed unevenly in separating two daughter cells in anaphase (left) or cytokinesis (right). Bars, 10 µm.