Actin filament homeostasis supports ring contraction in spheroplasts. (A) Schematic representation of the experimental design. (B) Formation of Rlc1-GFP clusters upon 100 µM latA treatment (10 rings). Asterisks, clusters. (C) Quantification of the number of clusters as a function of ring perimeter (27 rings), and quantification of the mean distance between clusters as a function of ring perimeter (27 rings). (D) Distribution of the distance between clusters (410 clusters). Red line, log-Gaussian fitted line to the distribution; R², fitting coefficient. The scattered dot plot shows all the distances between clusters. (E) Diagram illustrating the perturbation of actin turnover in spheroplasts by using latA and jasp. (F) Contraction of rings in spheroplasts after 100 µM latA and 100 µM jasp treatment (12 rings).