Figure 4.
Figure 4. Actin filament homeostasis supports ring contraction in cells. (A) S. japonicus cells expressing LifeAct-GFP were incubated with 100 µM latA (middle; 20 cells) or 100 µM latA and 100 µM jasp (bottom; 24 cells). DMSO was used as a control (top; 21 cells). (B) S. japonicus cells expressing Rlc1-GFP were incubated with 100 µM latA (middle; 24 cells) or 100 µM latA and 100 µM jasp (bottom; 30 cells). DMSO was used as a control (top; 21 cells). The rate of contraction (perimeter changes over time) was quantitated for cells incubated in DMSO (11 cells) or latA and jasp (11 cells). Box, 3D rotation of the ring at time point 18.7 min. (C) FRAP of Rlc1-GFP on the rings treated with latA and jasp. DMSO, seven cells; latA + jasp, 10 cells. (D) FRAP of Rlc1-GFP on the rings treated with latA alone in less than 10 min. DMSO, 10 cells; latA, 13 cells.

Actin filament homeostasis supports ring contraction in cells. (A) S. japonicus cells expressing LifeAct-GFP were incubated with 100 µM latA (middle; 20 cells) or 100 µM latA and 100 µM jasp (bottom; 24 cells). DMSO was used as a control (top; 21 cells). (B) S. japonicus cells expressing Rlc1-GFP were incubated with 100 µM latA (middle; 24 cells) or 100 µM latA and 100 µM jasp (bottom; 30 cells). DMSO was used as a control (top; 21 cells). The rate of contraction (perimeter changes over time) was quantitated for cells incubated in DMSO (11 cells) or latA and jasp (11 cells). Box, 3D rotation of the ring at time point 18.7 min. (C) FRAP of Rlc1-GFP on the rings treated with latA and jasp. DMSO, seven cells; latA + jasp, 10 cells. (D) FRAP of Rlc1-GFP on the rings treated with latA alone in less than 10 min. DMSO, 10 cells; latA, 13 cells.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal