Figure 5.
Figure 5. Ipl1/Aurora B is prematurely targeted to the metaphase spindle in she1Δ. (A) Spotting growth assays on rich media showing IPL1-3GFP is functional. (B) Ipl1-3GFP and mCherry-Tub1 in metaphase-arrested WT, she1Δ, dyn1Δ, and nip100Δ cells. (C) Quantification of spindle-associated Ipl1-3GFP intensity and percentage of cells with an enhanced Ipl1-3GFP phenotype during metaphase block. Error bars are SEM (top) and standard error of proportion (bottom). ***, P < 0.0001; n.s., P ≥ 0.01 by unpaired t test (n ≥ 94 for each). (D) Localization of Ipl1-3GFP in cells expressing the indicated she1 alleles. (E) Quantification of cells exhibiting diffused Ipl1-3GFP in the nucleus or enhanced Ipl1-3GFP on the spindle. Error bars are standard error of proportion.

Ipl1/Aurora B is prematurely targeted to the metaphase spindle in she1Δ. (A) Spotting growth assays on rich media showing IPL1-3GFP is functional. (B) Ipl1-3GFP and mCherry-Tub1 in metaphase-arrested WT, she1Δ, dyn1Δ, and nip100Δ cells. (C) Quantification of spindle-associated Ipl1-3GFP intensity and percentage of cells with an enhanced Ipl1-3GFP phenotype during metaphase block. Error bars are SEM (top) and standard error of proportion (bottom). ***, P < 0.0001; n.s., P ≥ 0.01 by unpaired t test (n ≥ 94 for each). (D) Localization of Ipl1-3GFP in cells expressing the indicated she1 alleles. (E) Quantification of cells exhibiting diffused Ipl1-3GFP in the nucleus or enhanced Ipl1-3GFP on the spindle. Error bars are standard error of proportion.

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