Figure 2. She1 deletion reduces... | Rockefeller University Press

Figure 2.

$Figure 2. She1 deletion reduces spindle-associated Bim1 and increases spindle MT dynamics. (A) Video frames showing bent spindle phenotype in MET3-CDC20 GFP-TUB1 she1-ΔN126 cells with cin8Δ, kip1Δ, kar3Δ, or dyn1Δ (n ≥ 50 cells). (B) Quantification and representative images of spindle-associated Cin8-3GFP, Ase1-3GFP, and Bim1-3mCherry in Cdc20-depleted she1Δ cells relative to WT (n ≥ 45 spindles). (C) Representative FRAP images of GFP-Tub1 spindles in WT and she1Δ cells arrested in metaphase. Photobleached areas are indicated by circles at 0 s. (D, top) Mean fractional recovery after photobleaching for 12 WT and 11 she1Δ spindles. Error bars are SEM. (Bottom) Quantification of half-maximal recovery time. P < 0.005 by unpaired t test.$

She1 deletion reduces spindle-associated Bim1 and increases spindle MT dynamics. (A) Video frames showing bent spindle phenotype in MET3-CDC20 GFP-TUB1 she1-ΔN126 cells with cin8Δ, kip1Δ, kar3Δ, or dyn1Δ (n ≥ 50 cells). (B) Quantification and representative images of spindle-associated Cin8-3GFP, Ase1-3GFP, and Bim1-3mCherry in Cdc20-depleted she1Δ cells relative to WT (n ≥ 45 spindles). (C) Representative FRAP images of GFP-Tub1 spindles in WT and she1Δ cells arrested in metaphase. Photobleached areas are indicated by circles at 0 s. (D, top) Mean fractional recovery after photobleaching for 12 WT and 11 she1Δ spindles. Error bars are SEM. (Bottom) Quantification of half-maximal recovery time. P < 0.005 by unpaired t test.