She1 is required for the maintenance of metaphase spindle stability. (A) Diagram of genomic she1 alleles and their ability to regulate cortical dynein activity. (B) Frequency of observing cells with enhanced dynein activity in each indicated strain (n ≥ 47 cells). n.s., not statistically significant, P > 0.1; ***, P < 0.0001 by unpaired t test. (C) Video frames showing spindle defects during metaphase arrest in MET3-CDC20 GFP-TUB1 cells. Frame boxed in red is shown in merge with Nuf2-mCherry in D. Right, graph showing frequency of observing indicated spindle phenotypes for each strain (n ≥ 80 cells each). Error bars in B and C are standard error of proportion. (D) Bending of ipMTs in metaphase-arrested WT, she1Δ, and she1-ΔN126 cells. KT, kinetochore; kMT, kinetochore MT. (E) Video frames showing recovery of a bent/collapsed spindle in she1Δ, she1-ΔN89, and she1-ΔN126 cells. Red or yellow indicates bent/collapsed or normal phenotype for each frame.