Membrane tether extraction experiment during P2. (A) Representative images of a membrane tether extraction. Yellow dashed lines, cell edge; white dashed line, region where the kymograph presented in B was generated. Bar, 5 µm. (B) Correlation between cell edge motion (kymograph) and tether force during four bucking cycles (I, II, III, and IV). Green dashed lines separate the buckling events as a function of edge position; blue dashed lines determine edge extension (in the kymograph) or variations in tension (in the curve) for each buckling event; plot in red, 2-s moving mean.