Figure 7. EBP-2 is required for... | Rockefeller University Press

Figure 7.

$Figure 7. EBP-2 is required for efficient force generation when dynein function is partially perturbed. (A) Embryonic lethality and brood size of dhc-1(or195ts) and dhc-1(or195ts); Δebp-2 strains at different temperatures shown as means ± SD. n = 4 animals each. (B) Quantification of centrosome velocities upon spindle severing. Bars show means ± SEM. Unpaired (between genotypes) and paired (within embryos) Student’s t tests; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. gfp::tbb-2 control, n = 25; ebp-2(RNAi), n = 21; dhc-1ts, n = 28; dhc-1ts; ebp-2(RNAi), n = 38. (C) Quantification of cortical MT residence time as described in Fig. 4 D. Bars are means ± SEM. Mann–Whitney U test; **, P < 0.01; ***, P < 0.001. n = 87–410 events from five embryos for each condition. Graphs show cumulative fractions of residence time distributions. Right graphs show density of cortical MT contacts as mean MTs/µm2 s−1 per embryo, and bars are means ± SEM. (D) Quantification of astral MT densities in metaphase embryos measured as shown in Fig. 4 F. Plots were aligned and normalized to maximum values at the centrosome. n = 20 asters from 10 embryos per condition. Bar, 1 µm.$

EBP-2 is required for efficient force generation when dynein function is partially perturbed. (A) Embryonic lethality and brood size of dhc-1(or195ts) and dhc-1(or195ts); Δebp-2 strains at different temperatures shown as means ± SD. n = 4 animals each. (B) Quantification of centrosome velocities upon spindle severing. Bars show means ± SEM. Unpaired (between genotypes) and paired (within embryos) Student’s t tests; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. gfp::tbb-2 control, n = 25; ebp-2(RNAi), n = 21; dhc-1ts, n = 28; dhc-1ts; ebp-2(RNAi), n = 38. (C) Quantification of cortical MT residence time as described in Fig. 4 D. Bars are means ± SEM. Mann–Whitney U test; **, P < 0.01; ***, P < 0.001. n = 87–410 events from five embryos for each condition. Graphs show cumulative fractions of residence time distributions. Right graphs show density of cortical MT contacts as mean MTs/µm² s⁻¹ per embryo, and bars are means ± SEM. (D) Quantification of astral MT densities in metaphase embryos measured as shown in Fig. 4 F. Plots were aligned and normalized to maximum values at the centrosome. n = 20 asters from 10 embryos per condition. Bar, 1 µm.