Analysis of dynein plus end tracking and development of Δebp mutants. (A) SDCM images illustrating eGFP::DHC-1 localization in WT, ebp-1/3(RNAi), and ebp-2(RNAi) embryos. Insets highlight the presence or absence of dynein comets. (B) ebp-1/3 and ebp-2 knockout strategies. Relevant genes are shown in blue and green, genetic duplication is in red, and genetic deletions are in gray. (C) SDCM images illustrating eGFP::DHC-1 localization in WT, Δebp-2, and Δebp-1/3 embryos. Insets highlight the presence or absence of dynein comets. (D) Embryonic lethality and brood size of Δebp mutants shown as means ± SD. n = 4 animals each. (E) DIC images of N2, Δebp-2, Δebp-1/3, and Δebp-1/2/3 one-cell embryos. Time past pronuclear meeting (t = 0) is indicated in seconds. Bar, 5 µm.