Dynein tracks MT plus ends during mitosis. (A and B) SDCM images of embryos expressing mCherry::DHC-1 and GFP::TBB-2 (A) or EBP-2::GFP (B). The arrow indicates dynein at the plus end. The white box indicates the zoomed image in C. (C) Intensity profiles of mCherry::DHC-1 and EBP-2::GFP comets shown as percentages of maximum. Means ± SD are shown. n = 10. (D) Kymographs (left) and mean velocities (right) of metaphase comets; mCherry::DHC-1 (n = 57) and EBP-2::GFP (n = 116) means ± SD. Unpaired Welch’s Student’s t test; not significant. (E and F) TIRF images of early anaphase mCherry::DHC-1 and GFP::TBB-2 (E) or EBP-2::GFP (F) localization. Graphs show intensity profiles as mean (n = 10) percentage of maximum ± SD. (G) Quantification of EBP-2::GFP and mCherry::DHC-1 colocalization as in F, shown as means ± SEM of per frame percentage colocalization of 2,351 mCherry::DHC-1 and 3,178 EBP-2::GFP particles from three embryos. (H) SDCM images of anaphase mCherry::DHC-1 and PH::eGFP localization. The leftmost three panels are kymographs of the invagination indicated in the third panel from the right (white dashed box). Graph shows intensity profiles as measured along the box in the rightmost panel, representative of n = 25 events. Bars: (horizontal) 1 µm; (vertical) 1 s.