Figure 1.
Figure 1. Endogenous tagging of dhc-1. (A) Dynein tagging strategies. Colors indicate dhc-1 exons (blue), mcherry (red), egfp (green), and linkers (orange). Cartoons illustrate dynein heavy chain with FP fused to the C (top) or N terminus (bottom). (B) Brood size and embryonic lethality of dhc-1 knock-in strains shown as means ± SD. n = 4 animals each. (C) Widefield image showing mCherry::DHC-1 expression in an adult with a zoom of the germline (red dashed box) below. Bars: (top) 50 µm; (bottom) 10 µm. (D) SDCM images of eGFP::DHC-1 localization in the one-cell embryo. Bar, 5 µm.

Endogenous tagging of dhc-1. (A) Dynein tagging strategies. Colors indicate dhc-1 exons (blue), mcherry (red), egfp (green), and linkers (orange). Cartoons illustrate dynein heavy chain with FP fused to the C (top) or N terminus (bottom). (B) Brood size and embryonic lethality of dhc-1 knock-in strains shown as means ± SD. n = 4 animals each. (C) Widefield image showing mCherry::DHC-1 expression in an adult with a zoom of the germline (red dashed box) below. Bars: (top) 50 µm; (bottom) 10 µm. (D) SDCM images of eGFP::DHC-1 localization in the one-cell embryo. Bar, 5 µm.

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