Sperm DNA is actively transported in pfn-1(RNAi). (A–C) Single frame (A) and time projection (B) from a time-lapse image of a control and pfn-1(RNAi) meiotic embryo expressing GFP::yolk and mCherry::histone. The asterisk marks the spindle DNA that was present for a few frames. Arrows point to sperm DNA. Bar, 10 µm. (C) Temporal color code to indicate sperm position through time. The scale is the same as in A and B. (D) Maximum (Max) 2D displacement of sperm DNA over a 30-s period. (E) Average and peak velocity of sperm DNA movement acquired at 5-s intervals. Average velocity: control = 0.06 ± 0.01 µm/s (n = 10); pfn-1(RNAi) = 0.11 ± 0.01 µm/s (n = 10). Peak velocity: control = 0.13 ± 0.01 µm/s (n = 10); pfn-1(RNAi) = 0.23 ± 0.02 µm/s (n = 10). (F and G) Average 3D MSD calculated from trajectories of sperm DNA plotted with linear (F) and logarithmic (G) axes. Images were acquired at a 5-s interval. Alpha for control = 1.06 (n = 10); alpha for pfn-1(RNAi) = 1.53 (n = 10). (H) Scatter plot of individual alphas from MSD plots of each time-lapse image captured used for analysis in F and G. n = 10. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; two-tailed t test. Error bars denote standard error of the mean.