F-actin is required for sperm DNA positioning. (A) Maximum intensity projections of control(RNAi)-, pfn-1(RNAi)–, or latrunculin A–treated fixed meiotic embryos. Asterisks indicate a polar body; arrows indicate sperm DNA location. Bar, 10 µm. (B) Sperm-to-spindle distance for meiotic embryos at all stages. Lat A, latrunculin A. Numbers are listed in Table S1. (C) Sperm-to-spindle distance for control and pfn-1(RNAi) meiotic embryos by stage. AI, anaphase I; AII, anaphase II, MI, metaphase I; MII, metaphase II. (D) Sperm-to–nearest cortex distance for embryos at all stages of meiosis. Numbers are listed in Table S1. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; two-tailed t test. Error bars denote standard error of the mean.