Figure 10.
Figure 10. Reduction of PTEN levels rescues glial scar formation inNestin-Stat3+/− mice. (A, left) Glial scar borders are poorly defined in Nestin-Stat3+/− mice at 21 dpi, in contrast to Nestin-Stat3+/−/Pten+/− that harbor scar borders similar to WT ones. Bar, 300 µm. (Right) Higher magnification view of the insets shown on left (nuclear staining by Hoechst in blue). Bar, 100 µm. (B) Glial scar borders were scored at 21 dpi. Reduction of PTEN level in Nestin-Stat3+/− genetic background rescues the formation of glial scar borders. (C) Immunostaining for fibronectin, a fibroblast marker, or CD11b, a marker of inflammatory cells, shows an easily delineated interface between the lesion center and the reactive astrocytes in WT and Nestin-Stat3+/−/Pten+/− mice, in contrast to the diffuse border in Nestin-Stat3+/− mice. Bars, 100 µm. Error bars indicate ±SEM. ***, P < 0.001.

Reduction of PTEN levels rescues glial scar formation inNestin-Stat3+/ − mice. (A, left) Glial scar borders are poorly defined in Nestin-Stat3+/− mice at 21 dpi, in contrast to Nestin-Stat3+/−/Pten+/− that harbor scar borders similar to WT ones. Bar, 300 µm. (Right) Higher magnification view of the insets shown on left (nuclear staining by Hoechst in blue). Bar, 100 µm. (B) Glial scar borders were scored at 21 dpi. Reduction of PTEN level in Nestin-Stat3+/− genetic background rescues the formation of glial scar borders. (C) Immunostaining for fibronectin, a fibroblast marker, or CD11b, a marker of inflammatory cells, shows an easily delineated interface between the lesion center and the reactive astrocytes in WT and Nestin-Stat3+/−/Pten+/− mice, in contrast to the diffuse border in Nestin-Stat3+/− mice. Bars, 100 µm. Error bars indicate ±SEM. ***, P < 0.001.

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