Expression of a Tuba1a S140G-mutant construct by electroporation in P0–P2 mouse pups impairs neuronal migration in the RMS. (A–C) Low-magnification images of GFP staining on sagittal brain sections after electroporation of GFP only (A), control WT Tuba1a (B), or Tuba1a S140G-mutant (C) ires GFP constructs at 5 dpe. (D–F) Higher magnifications of the OB are represented. Note the decreased number of GFP⁺ neurons in the OB from the brain electroporated with the mutant S140G construct. (G) Description of the three constructs used in this study: GFP only, control Tuba1a, and Tuba1a S140G constructs with GFP separated by an ires. The Dcx promoter controls expression. Schemas of the electroporation as well as the live-imaging system for slices from electroporated brains are shown. (H) Quantification of the percentage of GFP⁺ neurons per region (SVZ, RMS, and OB, as indicated in the schema) for GFP only, control Tuba1a, and Tuba1a S140G-mutant conditions. No significant differences were observed between GFP only and control Tuba1a conditions. Note the increase in the percentage of GFP⁺ neurons in the SVZ and the RMS but decrease in the OB for the S140G-mutant construct. *, P < 0.05; **, P < 0.01; ***, P < 0.001. n = 4–5. Data are represented as mean ± SEM. Bars: (A) 248 µm; (C) 64 µm.