Tda2 localizes to the actin cytoskeleton at sites of CME. (A) Live-cell fluorescence microscopy demonstrating strong colocalization between Tda2-GFP and Abp1-RFP. (B) When yeast cells were treated with latrunculin A (LatA), Tda2-GFP was not recruited to endocytic sites, whereas Sla1-RFP was recruited but remained largely immobile. White boxes indicate endocytic sites used for constructing kymographs on the right. Bars, 1 µm.