Tda2 belongs to the dynein light chain family. (A) Ribbon representation of the Tda2 crystal structure (PDB entry 5VKY) showing a homodimer via a combined central β sandwich, with each β sheet composed of four strands arranged in an antiparallel fashion and the two helices from each Tda2 molecule flanking the central β sandwich. One Tda2 molecule is shown in wheat color and the other in cyan, with some loops (magenta) on the cyan subunit being in poor density and omitted from the final model. Structure is rotated 90° for another view on the right. (B) Analytical ultracentrifugation data showing Tda2 to behave as a single species with a molecular weight of 30.8 kD, reflecting a dimer of 15.6-kD monomers in solution. (C and D) Cα-based superposition of Tda2 (wheat) and D. melanogaster TcTex1 (violet; PDB entry 1YGT, chain A) or S. cerevisiae LC8 (green; PDB entry 4HT6, chain E).