Figure 2.
Figure 2. Tda2 is needed for optimal endocytosis of a native cargo. (A) Endocytosis of Mup1-GFP was analyzed in WT (TDA2) and tda2Δ cells as described in the Fluorescence microscopy and endocytosis assays section of Materials and methods. Internalization of Mup1-GFP was delayed in tda2Δ cells. Representative images at indicated time points are pictured below the plots. (B) WT (TDA2), tda2Δ, and sla1Δ cells were incubated with the fluorescent dye Lucifer yellow and analyzed by fluorescence microscopy. Lucifer yellow internalization was modestly reduced in tda2Δ cells, whereas sla1Δ cells portray a severe defect. Vacuoles containing Lucifer yellow are seen after 2 h incubation, pictured below the plots. Bars, 10 µm. Error bars indicate means ± SEM. *, P < 0.05; **, P < 0.01.

Tda2 is needed for optimal endocytosis of a native cargo. (A) Endocytosis of Mup1-GFP was analyzed in WT (TDA2) and tda2Δ cells as described in the Fluorescence microscopy and endocytosis assays section of Materials and methods. Internalization of Mup1-GFP was delayed in tda2Δ cells. Representative images at indicated time points are pictured below the plots. (B) WT (TDA2), tda2Δ, and sla1Δ cells were incubated with the fluorescent dye Lucifer yellow and analyzed by fluorescence microscopy. Lucifer yellow internalization was modestly reduced in tda2Δ cells, whereas sla1Δ cells portray a severe defect. Vacuoles containing Lucifer yellow are seen after 2 h incubation, pictured below the plots. Bars, 10 µm. Error bars indicate means ± SEM. *, P < 0.05; **, P < 0.01.

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