Nuclear shape, internuclear distances, and LINC complex proteins were unaffected in TA muscles from nesprin-1ΔCH mice. (A) TA skeletal muscle fibers isolated from either WT or nesprin 1ΔCH (N1ΔCH) mice were fixed and stained using Lamin A/C (A/C), Lamin B1 (B1), Sun1, Sun2 (green), and α-actinin (red) antibodies. Arrowheads denote the specific localization to the NE. (B) Western blots of the LINC complex proteins Sun1 and Sun2, the LINC-associated protein emerin, and the nuclear lamins A/C, B1, and B2 from WT and nesprin 1ΔCH (KO) TA muscle lysates. Note that the localization and levels were unaffected in nesprin 1ΔCH compared with WT controls. GAPDH served as a loading control. (C) Internuclear distances were quantified in TA muscle fibers isolated from WT (blue bars) and nesprin 1ΔCH (green bars) mice shown in A. Note that the distances between nuclei were unaffected. n = 176–205 and n = 140–230 internuclear distances were counted for nesprin 1ΔCH and WT, respectively. (D) Low-magnification representative images of those shown in A. n = at least three to four pups per genotype for each panel. Bars: (A) 5 µm; (D) 10 µm. DIC, differential interference contrast.