Figure 4.
Figure 4. Dynein requires a functional motor to alter microtubule dynamics. (A) Diagram of dynein heavy chain, including the N-terminal tail domain, ATPase ring, and MTBD. Colored regions are tested in our analysis. (B) Representative images of cells expressing Dyn1-3GFP (yellow) and mRuby2-Tub1 (magenta). Each image is a maximum intensity projection from a confocal Z series. Arrowheads point to the different locations of dynein foci. Bars, 1 µm. (C) Median astral microtubule length in pre-anaphase cells. WT, n = 594 microtubules; dyn1Δ, n = 123; dyn1Δ-MTBD, n = 158 microtubules; dyn1-WA1, n = 175; dyn1-WA3, n = 184. Error bars are 95% c.i. (D) Mean dynamicity displayed in tubulin subunits per second. Data for WT and dyn1Δ from Fig. 2 E are included for reference. dyn1-WA1, n = 52 growth or shrinkage events; dyn1-WA3, n = 87. Error bars are SEM. Asterisks denote a significant difference from WT. **, P < 0.005; ****, P < 0.0001; determined by t test.

Dynein requires a functional motor to alter microtubule dynamics. (A) Diagram of dynein heavy chain, including the N-terminal tail domain, ATPase ring, and MTBD. Colored regions are tested in our analysis. (B) Representative images of cells expressing Dyn1-3GFP (yellow) and mRuby2-Tub1 (magenta). Each image is a maximum intensity projection from a confocal Z series. Arrowheads point to the different locations of dynein foci. Bars, 1 µm. (C) Median astral microtubule length in pre-anaphase cells. WT, n = 594 microtubules; dyn1Δ, n = 123; dyn1Δ-MTBD, n = 158 microtubules; dyn1-WA1, n = 175; dyn1-WA3, n = 184. Error bars are 95% c.i. (D) Mean dynamicity displayed in tubulin subunits per second. Data for WT and dyn1Δ from Fig. 2 E are included for reference. dyn1-WA1, n = 52 growth or shrinkage events; dyn1-WA3, n = 87. Error bars are SEM. Asterisks denote a significant difference from WT. **, P < 0.005; ****, P < 0.0001; determined by t test.

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