Mechanics-induced axonal varicosities are accompanied with transiently halted MT dynamics and axonal transport. (A) Kymograph of EB1-YFP plus end tracking before, during, and after puffing along an axonal segment. (B) Kymograph of Mito-YFP transport before, during, and after puffing along an axonal segment. (C) Kymograph of YFP-VAMP2 transport before, during, and after puffing along an axonal segment. Red arrowheads show movements before puffing, and blue arrowheads show movements after puffing. (D–F) Effects of puffing on moving velocity (D), distance (E), and frequency (F) of EB1-YFP plus end tracking, mitochondria transport, and presynaptic protein transport in anterograde and retrograde directions. Error bars indicate means ± SEM. Unpaired t test between pre- and post-puffing: *, P < 0.05; **, P < 0.01.