Large COPII vesicles colocalize with PC1. (A) PC1 (green) and KLHL12 (red) colocalize in KI6 cells that were treated with doxycycline for 7.5 h to induce the expression of KLHL12. Images shown are mean intensity projected views of a z-stack shown for each and merged channel. Red arrowheads indicate KLHL12-positive puncta, whereas white arrowheads indicate colocalized puncta. Rightmost panel shows 3D view of KLHL12 puncta obtained from the z-stack using the Spot feature in Imaris 8.1.2. An example of colocalizing yellow puncta is indicated by a white arrowhead, and KLHL12-positive puncta not colocalizing with PC1 is indicated by a red arrowhead. Total number of KLHL12-positive puncta determined in the z-stack was 27, and 78% of these spots were positive for PC1 calculated using CoLoc feature in Imaris 8.1.2. (B) PC1 (green), KLHL12 (magenta), and SEC31A (red) colocalize in KI6 cells (white arrowhead). DAPI is shown in blue. Half of the total number of KLHL12-positive puncta (n = 14) were positive for PC1 and SEC31A. (C) HSP47 (green), KLHL12 (red), and SEC31A (magenta) colocalize in KI6 cells (white arrowhead). Of the total number of KLHL12-positive puncta (n = 24) counted in 11 cells, 79% were positive for both SEC31 and HSP47. Bars, 10 µm. n = 3.