WASP is crucial for neutrophil motility. (A) Worm plots showing the tracks of cells migrating up an fMLP chemoattractant gradient. Control cells are on the left, and WASP-KD cells are on the right, with cells exhibiting the rhino phenotype in red. Cells were imaged for 20 min and migration paths were overlaid, with time 0 at (0,0). The endpoint of each cell’s path is shown with a dot. See also Video 2. (B) Depletion of WASP protein leads to reduced cell speed. The mean instantaneous speed for each cell in A is plotted as a dot color-coded by biological replicate to highlight the consistency from experiment to experiment. (C) Reduction of WASP protein leads to no significant change in directional persistence (the ratio of the Euclidean distance to the accumulated distance) of cells tracked in A. Means of the three replicates are displayed as horizontal lines; p-values were obtained from two-tailed paired t tests.