Abnormal chloroplast distribution in KCBP KO cells. (A) Snapshots of KCBP KO cells at 150 min after anaphase onset. green, chloroplast; magenta, histone-RFP. Bar, 50 µm. (B) Kymograph showing chloroplast distribution along the apical–basal axis throughout the cell cycle. White dotted lines indicate the position of the metaphase plate. Bars: (horizontal) 50 µm; (vertical) 120 min. (C) Chloroplast signal intensity was measured along cells’ long axes. Each cell was divided into four regions, and the mean signal intensity within each region was divided by the mean intensity of the whole cell. Mean relative values and SEMs are shown in this graph. Control, n = 8; KO line, n = 14; KO/KCBP-b line, n = 9. (D) Apical translocation of chloroplasts in KCBP KO. Arrowheads indicate tracking of two chloroplasts. Bars: (top) 20 µm; (bottom) 10 µm. (E) Net displacement of individual chloroplasts along a cell’s long axis in 30 min. Negative values were given when the chloroplast moved toward cell plate (basal direction). Mean and SD are marked (108 [control] or 68 [KO] chloroplasts in three cells; P < 0.0001; Mann–Whitney U test, two-tailed). (F and G) Directionality of EB1 signal motility during 60–90 min after anaphase onset (407 [control] or 235 [KO line] signals in five cells, mean ± SEM). A snapshot and kymograph are displayed. Bars: (top) 10 µm; (horizontal) 10 µm; (vertical in kymograph) 2 min.