Loss of gop-1 disrupts endosomal recruitment of UNC-108. (A and B) Time-lapse images of coelomocytes in wild type (WT; A) and gop-1(tm5384) (B) expressing GFP::UNC-108 and NUC-1::Cherry. 0 min, time point before GFP::UNC-108 was initially detected on endosomes. White arrowheads, recruitment of GFP::UNC-108 to the endosome membrane; blue arrowheads, appearance of NUC-1::Cherry; yellow arrowheads, absence of UNC-108 from endosomes in gop-1(tm5384). The duration of UNC-108 on endosomes was quantified and is shown beneath the images (mean ± SD). n, number of endosomes quantified. (C and D) DIC and fluorescent images of coelomocytes in wild type (WT; C and C′) and gop-1(tm5384) (D and D′) expressing GOP-1::GFP. White arrowheads, GOP-1::GFP-positive vesicles. (E) Percentage of animals that contain GOP-1::GFP-positive vacuoles in coelomocytes was quantified and is shown. n, number of worms that were quantified. Bars, 5 µm.