Loss of gop-1 affects endosome and dense core vesicle maturation. (A–J) DIC and fluorescent images of coelomocytes expressing RME-8::GFP (A–D′) or LMP-1::GFP (E–H′) in the indicated strains. RME-8::GFP and LMP-1::GFP associate with endosomes and lysosomes, respectively, in wild type (white arrowheads), but label enlarged vacuoles (yellow arrowheads) in gop-1(tm5384), unc-108(n3263), and unc-108;gop-1 worms. Quantification is shown in I and J. At least 30 animals were scored in each strain. (K–O) Confocal fluorescence images of the dorsal nerve cord in the indicated strains expressing NLP-21::VENUS (K–N). Quantification is shown in O. At least 30 animals were scored in each strain. Data are shown as mean ± SD. One-way ANOVA with Tukey’s posttest was performed to compare mutant datasets with wild type or datasets linked by lines. **, P < 0.0001; N.S., no significance. (P–T) Confocal fluorescence images of the neuronal cell body in the ventral nerve cord expressing NLP-21::VENUS in the indicated strains. White arrowheads, small vesicles (<0.4 µm²); yellow arrowheads, large ones (>0.8 µm²). The surface area of NLP-21–positive vesicles in neuronal bodies was quantified in the indicated strains, and the size distribution of vesicles is shown in T. Purple lines indicate the mean surface area. At least 15 animals were scored in each strain. One-way ANOVA with Tukey’s posttest was performed to compare mutant datasets with wild type or datasets linked by lines. **, P < 0.0001; N.S., no significance. Bars: (A–H′ and K–N) 5 µm; (P–S) 2 µm.