Recruitment of GOP-1 to phagosomes requires RAB-5 but not RAB-7 function. (A–C″) Confocal fluorescence images of the sheath cell in wild type coexpressing GFP::GOP-1 and Cherry::UNC-108 (A–A″) or Cherry::FAPP1-PH (B–B″, trans-Golgi marker) or coexpressing GFP::UNC-108 and Cherry::FAPP1-PH (C–C″). White arrowheads, vesicles containing overlapping GFP and Cherry fluorescence; pink arrowheads, GFP- and Cherry-positive vesicles that are in close proximity; yellow arrowheads, GFP-positive and Cherry-negative puncta. Quantification is shown at right. At least 10 animals were scored in each strain. Data are shown as mean ± SD. (D–I′) DIC and fluorescence images of embryos expressing GFP::GOP-1 in the indicated strains. White arrowheads, cell corpses labeled by GFP::GOP-1; yellow arrowheads, unlabeled ones. (J and K) Quantification of somatic (J) and germ cell (K) corpses labeled by GFP::GOP-1 in the indicated strains is shown. At least 15 animals were scored in each strain. Data are shown as mean ± SD. Recruitment of GOP-1 in unc-108(n3263) and tbc-2(qx20) was compared with that in wild type, whereas data derived from rab-5 RNAi or rab-7 RNAi were compared with the control group by unpaired t tests. **, P < 0.0001. All other points had P > 0.05. Bars, 5 µm.