Figure 4.

Loss of gop-1 and unc-108 affects phagosomal association of RAB-7 and phagolysosome formation. Time-lapse images of somatic cell corpses in wild type (WT), unc-108(n3263), and gop-1(tm5384) embryos expressing GFP::RAB-7 (A) or coexpressing mCherry::MTM-1(C378S) and LAAT-1::GFP (B–F). 0 min, time point before RAB-7 or MTM-1 was first detected around the cell corpse; yellow arrowheads, MTM-1 release from phagosomes; white arrowheads, shrinkage of RAB-7– or LAAT-1–positive phagosomes. The percentage of phagosomes with the representative pattern is shown at left (A) or top (B–F). Quantification of RAB-7 duration on phagosomes (A) or the time between MTM-1 release and LAAT-1 recruitment is shown beneath the images (mean ± SD). n, number of phagosomes quantified. Bars, 2.5 µm.

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