Figure 3.
Loss of gop-1 and unc-108 affects the dynamics of RAB-5 and PtdIns3P on phagosomes. Time-lapse images of somatic cell corpses in wild type (WT), gop-1(tm5384), unc-108(n3263), tbc-2(qx20), and unc-108;tbc-2 embryos expressing GFP::RAB-5 (A) or YFP::2xFYVE (B). 0 min, time point before RAB-5 or FYVE was first detected around the cell corpse; arrowheads, time point when GFP::RAB-5 or YFP::2xFYVE disappears from phagosomes. Bars, 2.5 µm. Quantifications are shown in C and D. Data are shown as mean ± SD. One-way ANOVA with Tukey’s posttest was performed to compare mutant datasets with wild type. **, P < 0.0001.