gop-1and unc-108 act in the same pathway to regulate phagosome maturation. (A and B) Percentage of somatic (A) and germ cell corpses (B) labeled by various phagosomal markers at the 1.5-fold (A) or adult (48 h after L4/adult molt; B) stage was quantified in the indicated strains. (C–P) DIC and fluorescent images of the gonads stained by Lysosensor green (C–H′) or expressing LAAT-1::GFP (J–O′) in the indicated strains. White arrowheads, cell corpses labeled by Lysosensor green or LAAT-1::GFP; yellow arrowheads and boxes, unlabeled corpses. Quantification is shown in I and P. In A, B, I, and P, at least 15 animals were scored in each strain, and data are shown as mean ± SD. In A and B, data derived from different genetic backgrounds were compared by two-way ANOVA followed by Bonferroni posttest. *, P < 0.05; **, P < 0.001. All other points had P > 0.05. In I and P, one-way ANOVA with Tukey’s posttest was performed to compare mutant datasets with wild type or datasets linked by lines. **, P < 0.0001. Bars, 5 µm.