Information encoded in a wound-healing experiment. (A) Reducing a wound-healing experiment to a 12-dimensional feature vector encoding spatiotemporal dynamics. (B) Speed feature vectors for 402 control experiments. The bottom panel displays the normalized features. (C) Association of principal components (PCs) 1–3 with wound-healing rate. corr, correlation. (D) Coefficients of PCs 1–3, together encoding 92% of the speed variance in control experiments. (E) Effect on PCs by depletion of Cdc42, Rac1, and RhoA. Statistics via Wilcoxon signed rank test: *, P ≤ 0.01; **, P ≤ 0.001; ***, P ≤ 0.0001.