Figure 4.
Figure 4. Localization of caspase-2 activation platforms within the nucleolus. (A) HeLa.C2 Pro-BiFC cells transfected with Fibrillarin-CFP (2 µg), TFP-Nucleolin (NCL; 2 µg), or TFP-NPM1 (2 µg) were treated with or without camptothecin (10 µM) plus qVD-OPH (20 µM). Representative images show cells (red) with caspase-2 BiFC (yellow) and the indicated nucleolar proteins (blue). Bars, 10 µm. 3D reconstructions of the white boxed region composed from 0.2-µm serial confocal images through the z-plane of the cell are shown as orthogonal-slice views (right). The middle, right, and top panels are the xy, yz, and xz planes, respectively. The yz and xz planes intersect according to the crosshairs. Bars, 2 µm. (B) 3D graphs of pixel intensities of TFP-NPM1 and C2-BiFC signals in a representative untreated and camptothecin-treated cell.

Localization of caspase-2 activation platforms within the nucleolus. (A) HeLa.C2 Pro-BiFC cells transfected with Fibrillarin-CFP (2 µg), TFP-Nucleolin (NCL; 2 µg), or TFP-NPM1 (2 µg) were treated with or without camptothecin (10 µM) plus qVD-OPH (20 µM). Representative images show cells (red) with caspase-2 BiFC (yellow) and the indicated nucleolar proteins (blue). Bars, 10 µm. 3D reconstructions of the white boxed region composed from 0.2-µm serial confocal images through the z-plane of the cell are shown as orthogonal-slice views (right). The middle, right, and top panels are the xy, yz, and xz planes, respectively. The yz and xz planes intersect according to the crosshairs. Bars, 2 µm. (B) 3D graphs of pixel intensities of TFP-NPM1 and C2-BiFC signals in a representative untreated and camptothecin-treated cell.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal