Distribution of cytoplasmic IFs in astrocytes. (A) Epifluorescence image of nestin (green), vimentin (magenta), and GFAP (yellow) immunostaining of a migrating astrocyte 10 h after wounding. The intensity profiles were obtained along the dotted arrow. Bars, 20 µm. (B) 3D structured illumination microscopy images of nestin (green), vimentin (magenta), and GFAP (yellow) immunostaining at the front of a migrating astrocyte. Kinesin depletion was used to decrease the density of IFs at the cell front, facilitating the visualization of single filaments. A higher magnification of the boxed region is shown in the middle. Fluorescence (Fluo.) intensity profiles showing the distribution of nestin, vimentin, and GFAP along a single filament (indicated by a dotted arrow in the inset) are shown on the right. Bars: (main image) 10 µm; (inset) 1 µm. a.u., arbitrary units.